Figure 2: Unique amino-acid sequences of β5t-dependent peptides generated in vitro.
From: Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells
Substrate proteins were digested by purified iCPs and tCPs in vitro. Identified cleavage sites generated specifically by the iCPs (blue) and by the tCPs (red) were compared. Amino-acid frequencies at P1–P5 and P1′ are shown. Grey bars depict amino-acid compositions of the substrates. In vitro digestion assay was repeated at least three times.
