Figure 6: OVAp peptide variants with tCP-dependent motifs show lower affinities to OT-I TCR.
From: Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells
(a) List of OVAp variants used for assays in b–d and Fig. 7a,b. (b) Binding of peptide/H-2Kb tetramers to CD4/CD8 double-positive thymocytes from Tap1−/− OT-I TCR-transgenic mice. (c) Dulling of CD4/CD8 co-receptors in OT-I thymocytes by OVAp variants. Thymocytes from Tap1−/− OT-I TCR-transgenic mice (n=3) were stimulated in the presence of peptides at indicated concentrations and irradiated splenocytes from B6-Ly5.1 mice. (d) Activation of mature OT-I cells by OVAp variants. Splenocytes from Rag2−/− OT-I TCR transgenic mice (n=3) were cultured in the presence of peptides at indicated concentrations for 5 h. Representative data from three (b) and four (c,d) independent experiments.
