Figure 7: OVAp peptide variants with tCP-dependent motifs promote the positive selection of OT-I CD8+ T cells.
From: Thymoproteasomes produce unique peptide motifs for positive selection of CD8+ T cells
(a) FTOC was performed using E15 thymus lobes from Tap1−/− OT-I TCR transgenic mice in the presence of the indicated peptides (listed in Fig. 6a). Three days after the beginning of the culture, cells were harvested and analysed using flow cytometry. Representative CD4 versus CD8 plots from three independent experiments and (b) average numbers±s.e.m. (n=3) of Vα2high CD8 single-positive (SP) thymocytes per thymus lobe are shown. Cumulative data of three to four thymus lobes per experimental group are shown in b. ***P<0.01, **P<0.01, *P<0.05, N.S. not significant in comparison with the cell number from the culture in the presence of gp33 peptide. Statistical analyses were performed by Student’s t-test.
