Figure 1: Biliary diversion schematic and effects on body weight, food intake and body composition.
From: Bile diversion to the distal small intestine has comparable metabolic benefits to bariatric surgery
(a) For the biliary diversion procedure, the common bile duct was ligated proximal to the pancreatic duct. The gallbladder was then anastomosed to one of the following: (1) jejunum 4 cm distal to the ligament of Treitz (GB-J), (2) ileum 4 cm proximal to the ileo-caecal valve (GB-IL) or (3) gallbladder-duodenal anastomosis (GB-D model) at the level of the ampulla of Vater. GB-D was performed without significant alteration of bile flow and functioned as a sham surgery. The RYGB procedure was performed as we previously described31. Mice were fed a high fat diet (HFD) for induction of diet-induced obesity (DIO), underwent the surgical procedures and were monitored for 8 weeks post-operatively. (b) Average daily food intake, Bio-Serv F3282 (5.49 kcal g−1); (c) relative change in body weight in N of 15 DIO, 15 GB-IL and 7 RYGB mice; and (d) serial body composition measures via NMR of fat and lean mass (N of 15 DIO, 12 GB-D, 11 GB-J, 15 GB-IL, 12 RYGB). Values shown are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001 versus DIO controls by one-way analysis of variance with Dunn’s post-test.
