Figure 1: Hepatic SNAT2 expression activates mTORC1/S6K pathway and induces hypertriglyceridemia. | Nature Communications

Figure 1: Hepatic SNAT2 expression activates mTORC1/S6K pathway and induces hypertriglyceridemia.

From: A hepatic amino acid/mTOR/S6K-dependent signalling pathway modulates systemic lipid metabolism via neuronal signals

Figure 1

(a) Hepatic total AA concentrations were measured in 8-week-old lean (white bar) or DIO (grey bar) and ob/ob (black bar) mice (n=5–7). (b–h) SNAT2 (black bars) or control LacZ (white bars) adenovirus was administered to high-fat diet-fed C57BL/6 mice (b, c and f–h), and standard chow-fed C57BL/6 mice (d and e). (b) Total AA concentrations in the liver were measured (n=5). (c) Immunoblotting of liver extracts with anti-phospho-mTOR, mTOR, phospho-p70-S6K, p70-S6K, phospho-S6 and S6 antibodies. The representative images derived from at least two experiments were displayed. (d and g) Serum TG levels under 10 h-fasted or -fed conditions were measured (d; n=5–7, g; n=5–7) and (e and h) HPLC analyses of sera in fed states were performed on day 5 after adenovirus administration (e; n=6–7, h; n=5–6). (f) Photograph of sera obtained from controls (left) and SNAT2 mice (right) fed a high-fat diet. Data are presented as means±s.d. *P<0.05, **P<0.01 by the unpaired t-test.

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