Figure 2: Suppression of plasma TG-hydrolysis activity induces hypertriglyceridemia. | Nature Communications

Figure 2: Suppression of plasma TG-hydrolysis activity induces hypertriglyceridemia.

From: A hepatic amino acid/mTOR/S6K-dependent signalling pathway modulates systemic lipid metabolism via neuronal signals

Figure 2

SNAT2 (black bars) or control LacZ (white bars) adenovirus was administered to standard chow-fed C57BL/6 mice (a–d), or high-fat diet-fed C57BL/6 mice (e–i). (a and f) Food intakes were measured for 5 days after adenovirus administration (a; n=11, f; n=5–6). (b and e) The rates of hepatic TG secretion were determined (b; n=5–6, e; n=9–11). (c and g) Fat-tolerance tests were performed (c; n=4–8, g; n=3–7). (d and h) TG-clearance studies were performed (d; n=5–11, h; n=5–6). (i) Plasma TG-hydrolysis activities were measured after the injection of heparin into the tail veins of 4 h-fasted mice (n=4–5). (j–l) Vehicle (white bars) or orlistat (black bars) was administered into the intraperitoneal space of C57BL/6 mice fed standard chow. Plasma TG-hydrolysis activities (j) were measured 90 min after the injection of these agents (n=7–8). Next, serum TG levels (k) were measured, and HPLC analyses of sera from these mice (l) were performed (k; n=5, l; n=5). (m–o) Vehicle (white bars) or orlistat (black bars) was administered into the intraperitoneal space of C57BL/6 mice fed a high-fat diet. (m) Photograph of sera obtained from vehicle- (left) and orlistat-administered mice (right). Serum TG levels (n) were measured, and HPLC analyses of sera from these mice (o) were performed (n; n=4–5, o; n=4). (p–s) SNAT2 (black bars) or control LacZ (white bars) adenovirus was administered to high-fat diet-fed C57BL/6 mice. (p) TG-hydrolysis activities were measured at either a normal or a high NaCl concentration after the injection of heparin into the tail veins of 4 h-fasted mice (n=6). (q) LPL mRNA expressions in WAT were analysed by RT–PCR (n=5–7). (r) Immunoblotting of WAT extracts with anti-LPL antibody. The representative images derived from at least two experiments were displayed. (s) TG-hydrolysis activity in WAT was examined on day 5 after adenovirus administration (n=5). Data are presented as means±s.d. *P<0.05, **P<0.01 by the unpaired t-test. NS, not significant.

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