Figure 2: Indirect topology assay of EmrE-C+ and HA-EmrE-C+. | Nature Communications

Figure 2: Indirect topology assay of EmrE-C+ and HA-EmrE-C+.

From: Dual-topology insertion of a dual-topology membrane protein

Figure 2

(a) Diagram of the EmrE constructs used. (b) The growth of 10-fold dilutions of stationary phase cell culture expressing the HA-EmrE-C+ or EmrE-C+ constructs with a single topology EmrE mutant (HA-EmrElocked_in or HA-EmrElocked_out), spotted on agar plates in the presence of 190 μg ml−1 ethidium bromide.

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