Table 1 Primer sequences.
From: The matrix protein Fibulin-5 is at the interface of tissue stiffness and inflammation in fibrosis
Gene | 5′ primer sequence | 3′ primer sequence |
|---|---|---|
β-actin | GGGCTATGCTCTCCCTCAC | GATGTCACGCACGATTTCC |
IL-1α | CTCTAGAGCACCATGCTACAGAC | TGGAATCCAGGGGAAACACTG |
IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
RANTES | CCTCACCATCATCCTCACTGCA | TCTTCTCTGGGTTGGCACACAC |
IκBα | GGAGCGCTTGGTGGACGATC | GCCCTGCTCACAGGCAAGAT |
S100a8 | AAATCACCATGCCCTCTACAAG | CCCACTTTTATCACCATCGCAA |
S100a9 | ATACTCTAGGAAGGAAGGACACC | TCCATGATGTCATTTATGAGGGC |
α-SMA | ATCGTCCACCGCAAATGC | AAGGAACTGGAGGCGCTG |
SPARC | GCTGTGTTGGAAACGGAGTTG | CTTGCCATGTGGGTTCTGACT |
CTGF | GTGCCAGAACGCACACTG | CCCCGGTTACACTCCAAA |
col1a1 | GCCAAGAAGACATCCCTGAAG | TCATTGCATTGCACGTCATC |
HSP90a | CAGTGATGATGAGGCTGAAG | CAGGATTTCTCGTCCAAATCG |
FSP-1 | CAGCACTTCCTCTCTCTTGG | TTTGTGGAAGGTGGACACAA |
CXCL5 | AGCTCGCCATTCATGCGGAT | CACTGCGAGTGCATTCCGCT |
CXCL10 | GTCATTTTCTGCCTCATCCTGCT | GGATTCAGACATCTCTGCTCATCA |
CXCL16 | CCTTGTCTCTTGCGTTCTTCC | TCCAAAGTACCCTGCGGTATC |
NLRP3 | ATTACCCGCCCGAGAAAGG | TCGCAGCAAAGATCCACACAG |
CRP | ATGGAGAAGCTACTCTGGTGC | ACACACAGTAAAGGTGTTCAGTG |
p53 | CTCTCCCCCGCAAAAGAAAAA | CGGAACATCTCGAAGCGTTTA |
IL-6 | CGTGGAAATGAGAAAAGAGTTGTG | CCAGTTTGGTAGCATCCATCATTTCT |
col3a1 | CTGTAACATGGAAACTGGGGAAA | CCATAGCTGAACTGAAAACCACC |
col6a2 | AAGGCCCCATTGGATTCCC | CTCCCTTCCGACCATCCGAT |
TGF-β1 | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
TGF-β2 | CTTCGACGTGACAGACGCT | GCAGGGGCAGTGTAAACTTATT |
TGF-β3 | CCTGGCCCTGCTGAACTTG | TTGATGTGGCCGAAGTCCAAC |
ANLN | GTTAAAACTCGAATGCAAAGGCT | AGTTGGCACTGGTGCAAAGTA |
DIAPH3 | GAGAAGCGACCCAAGTTGCAT | GAAGGGGAGGTCTCTCTTTCTT |
Fibulin-5(human) | CTACTCGAACCCCTACTCGAC | TCGTGGGATAGTTTGGAGCTG |
