Figure 3: Oligonucleotide quality.
From: Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries
PAGE analysis of three different subpools (A, B and C) synthesized by enzymes (enz) or else chemically (syn). Lane 1: crude product of final double-nicking reaction. Bands marked in green are staple strands, bands marked in blue are undesired concatemers (c), the intervening sequence (i) and the excess of two nicking primers (n) and can be removed well using HPLC purification (lane 2). Lanes 2, 4 and 6 are HPLC-purified c2ca reactions. Lanes 3, 5 and 7 contain unpurified synthetic oligonucleotides from a commercial vendor. M is a size marker.
