Figure 2: Confirmation of the TeA biosynthetic gene and domain structure.
From: Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS–PKS hybrid enzyme
(a) UPLC analysis of metabolites extracted from WT, WT+DMSO, ΔMGG_07803+DMSO and a TAS1 overexpression strain (OE::TAS1) without DMSO. Each strain was static-cultured for 5 days at 25 °C. Arrows indicate TeA peak. A 100 μM TeA standard (TeA ST) was also analysed. (b) Domain structure of TAS1 and typical fungal PKS–NRPS hybrid enzyme; blue indicate the NRPS portion, and yellow indicates the PKS portion.
