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MicroRNA expression profiling using LNA-modified probes in a liquid-phase bead-based array

Nature Methods volume 4, page 462 (2007)Cite this article

Abstract

Here we describe a new liquid-phase, bead-based array for in-solution expression analysis of microRNAs (miRNAs). The array combines Locked Nucleic Acid (LNA™)-modified capture probes and the xMAP® multiplexing bead technology. Incorporation of LNA in the array capture probes greatly increases their affinity for their short miRNA targets, thereby adding selectivity to the array. As a result, the FlexmiR™ system offers a highly specific, robust and fast miRNA profiling platform.

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Figure 1
Figure 2: Single-mismatch discrimination ability of the FlexmiR system.
Figure 3: Unsupervised hierarchical clustering and heat map showing that three human tissues clearly segregate from one another based on their miRNA expression profiles.

References

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Acknowledgements

We thank the Luminex Bioscience Group for kindly providing the data included in Figure 2.

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Authors and Affiliations

  1. Exiqon A/S, Bygstubben 9, Vedbaek, DK-2950, Denmark

    Anna Karina Busch, Thomas Litman & Peter Stein Nielsen

Authors
  1. Anna Karina Busch
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  2. Thomas Litman
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  3. Peter Stein Nielsen
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Corresponding author

Correspondence to Anna Karina Busch.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Busch, A., Litman, T. & Nielsen, P. MicroRNA expression profiling using LNA-modified probes in a liquid-phase bead-based array. Nat Methods 4, 462 (2007). https://doi.org/10.1038/nmeth1045

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