Supplementary Figure 1: Detailed description of setup of pressure monitoring system.
From: Intubation-based anesthesia for long-term time-lapse imaging of adult zebrafish
To be assembled by an electrician
Housing small distribution board AK03, Part#73540301, manufacturer: Spelsberg AK A2.2 Schuko plug, Part# 10754, manufacturer Mennekes, non-metallic sheathed cable 3 way NYM-J 32x1,5 T2.3 power supply for DIN-rails, 120 - 370 V ac, 90-265 V ac, 24Vdc 0,42A, Part# 1SVR427041R0000 - CP-D 24/0.42, manufacturer: ABB K2.5 monostable relay for DIN-rails, 24Vdc/1,25W coil, two way closer, 20A/250Vac, Part#: 22.22.9.024.4000 manufacturer: Finder K2.6 monostable relay for DIN-rails, 24Vdc coil, two way opener, 20A/250Vac, Part#: 22.24.9.024.4000 manufacturer: Finder P2.4 pressure switch, Part#DS-E1V10F4B2PR00, manufacturer: Norgren, connector plug M12x1, Part# 0523057, manufacturer: Norgren A2.7 Schuko wall socket; Part# 620W, manufacturer: Jung, non-metallic sheathed cable 3 way NYM-J 32x1,5 Explanation of setup: A2.2 provides 230V AC power input. T2.3 supplies P2.4, K2.5 and K2.6 with 24V DC. In the case the monitored pressure is lower than switching point 1 (SP1), K2.5 closes activating output of 230 V AC at A2.7. K2.5 opens again deactivating output of 230 V AC at A2.7 as the monitored pressure is below re-switching point 1 (rP1). This ensures the peristaltic pump plugged into A2.7 is only active when the vacuum pump is active. In case of vacuum pump failure, A2.7 has no output power and the peristaltic pump stops. In case the monitored pressure is lower than SP2, K2.6 opens deactivating output of 230 V AC at A2.7. K2.6 closes again activating output of 230 V AC at A2.7 as the monitored pressure is below rP2. This ensures that the peristaltic pump is switched off in case the soaking tubing to remove surplus of water in the mold is blocked e.g. by a piece of agarose.
