NRMT organizes methyl transfer

Wrighton, Katharine H.

doi:10.1038/nrm2964

Research Highlight
Published: 18 August 2010

Post-translational modification

NRMT organizes methyl transfer

Katharine H. Wrighton

Nature Reviews Molecular Cell Biology volume 11, page 605 (2010) Cite this article

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“NRMT is an α-N-methyltransferase for RCC1.”

The authors assessed HeLa cell nuclear fractions for protein methylation and analysed the fraction that methylated RCC1 using mass spectrometry. They identified an uncharacterized methyltransferase called METTL11a, a member of the methyltransferase 11 family, which they rename NRMT. Overexpression of NRMT in cells increases RCC1 α-N-methylation on the N-terminal Ser, whereas depleting NRMT decreases this, confirming that NRMT is an α-N-methyltransferase for RCC1. The authors sought to gain insight into the function of this modification by examining the distribution of RCC1 in NRMT-depleted cells. Less RCC1 is associated with chromatin in cells lacking NRMT, and NRMT-depleted cells in mitosis exhibit ∼3 times more supernumerary spindles than controls. These data suggest that RCC1 α-N-methylation stabilizes its association with chromatin to ensure proper mitotic division.

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ORIGINAL RESEARCH PAPER

Schaner Tooley, C. E. et al. NRMT is an α-N-methyltransferase that methylates RCC1 and retinoblastoma protein. Nature 28 July 2010 (doi: 10.1038/nature09343)

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