Abstract
Aim: To assess the effects of tracheal occlusion (TO) on pulmonary myofibroblast and matrix markers, TGF-β superfamily, and epithelial maturation in a newborn model of diaphragmatic hernia (CDH).
Methods: On day 23/31, rabbit fetuses were randomized to CDH or SHAM thoracotomy. On day 28/31, the same fetuses randomly underwent TO or SHAM-TO. After ventilation at term, lungs were harvested for RTq-PCR. Non-operated littermates served as controls. Gene expressions in left lungs were expressed as fold induction relative to controls.
Results: TO increased significantly LBWR in CDH and SHAM-CDH animals. Data as mean±SD. Difference with CDH-SHAM TO (*) or SHAM CDH-SHAM TO (+) for p< 0.05 (Kruskal-Wallis or one-way ANOVA).
Conclusion: TO enhanced transcription of markers of myofibroblast and alveolar type II cell differentiation, together with an overexpression of TGF-β isoforms. These data suggest that TO might induce matrix formation and tends to inhibit surfactant synthesis under the action of TGF-β.
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Vuckovic, A., Herber-Jonat, S., Rühl, I. et al. Markers of Extracellular Matrix and Epithelial Cell Maturation after Fetal Tracheal Occlusion in Newborn Rabbits with Lung Hypoplasia Induced by Diaphragmatic Hernia. Pediatr Res 70 (Suppl 5), 564 (2011). https://doi.org/10.1038/pr.2011.789
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DOI: https://doi.org/10.1038/pr.2011.789
