Fig. 2: Rational design of circRNA sequences. | Experimental & Molecular Medicine

Fig. 2: Rational design of circRNA sequences.

From: Optimal design of synthetic circular RNAs

Fig. 2

Each factor to be considered is presented for each region of the vector of interest. Spacers were added to lessen the structural hindrance between the IRES and the gene of interest or the splice junction. Adding motifs for RBPs that enhance translation is beneficial. Several IRESs with stronger activity than the canonically used IRESs and additional codon optimization can result in faster and more abundant protein production.

Back to article page