Fig. 1: m6A modification landscape in monocyte-derived macrophages.
Monocytes were stimulated with GM-CSF to induce macrophage differentiation. MeRIP-seq was performed using monocytes incubated with GM-CSF for 0 days, 3 days and 5 days (0D, 3D and 5D, respectively). a, The distribution of m6A peaks in gene body regions, including the 5′ UTR, CDS and 3′ UTR, among the three groups. b, The density of differential m6A peaks was compared between the indicated groups. c, Bar plot showing the number of mRNAs containing m6A sites in the three groups. d, Venn diagram showing the number of shared genes detected between the indicated groups. e, KEGG pathway enrichment analysis of DEGs was compared between the indicated groups. f,g, Correlation analysis of gene expression levels and changes in m6A modifications in the 3D_vs_0D group (f) or in the 5D_vs_3D group (g). The x axis denotes the change in mRNA (RNA-seq); the y axis denotes the change in m6A peaks (RIP-seq). h, Venn diagram showing the number of shared genes detected from RNA-seq and RIP-seq between the indicated groups. i, Heatmap showing the differences in expression and m6A modifications between the 3D and 0D groups or between the 5D and 3D groups.
