Fig. 3: KDM6B regulates lipid uptake, ROS production and IFN-γ-induced STAT1 phosphorylation in macrophages.

a, Macrophages were pretreated with GSKJ1 for 30 min, stimulated with IFN-γ for 12 h and subsequently incubated with oxLDL for 24 h. Lipid uptake was evaluated via Oil Red O staining. Scale bars, 200 μm (top) and 100 μm (bottom). b, Macrophages were pretreated with GSKJ1 for 30 min and then stimulated with IFN-γ for 12 h. Mitochondrial ROS production was detected via a ROS assay kit. Green fluorescence indicates the intensity of the ROS. Scale bar, 100 μm. The macrophages were pretreated with GSKJ1 for 30 min and then stimulated with IFN-γ for 12 h, and total RNA was subsequently collected for RNA-seq. c, Cnet plot showing KEGG enrichment pathways identified from DEGs between the IFN-γ-treated group and the GSKJ1 + IFN-γ-treated group. d, Heatmap showing DEGs among the control, IFN-γ and GSKJ1 + IFN-γ groups. e, Heatmap showing the enriched metabolic pathways affected by GSKJ1 treatment in macrophages, with differentially regulated pathways marked in red. f, Left: western blot analysis of Stat1, p-Stat1 and Kdm6b in macrophages pretreated with GSKJ1 for 30 min before they were stimulated with IFN-γ at the indicated times, with Gapdh used as an endogenous control. Right: the quantified results. *P < 0.05.