Fig. 4: KDM6B facilitates IFN-γ signaling by interacting with Jak1 and increasing its phosphorylation. | Experimental & Molecular Medicine

Fig. 4: KDM6B facilitates IFN-γ signaling by interacting with Jak1 and increasing its phosphorylation.

From: METTL3/RBM15 augments the stability of Kdm6b mRNA and promotes STAT1-mediated macrophage activation and atherosclerosis

Fig. 4: KDM6B facilitates IFN-γ signaling by interacting with Jak1 and increasing its phosphorylation.The alternative text for this image may have been generated using AI.

a, Macrophages were pretreated with GSKJ1 for 30 min and then stimulated with IFN-γ at the indicated times. Left: western blot analysis of Stat1, p-Stat1 and Kdm6b was performed, with Gapdh used as an endogenous control. Right: the quantified results. *P < 0.05. b, Macrophages were pretreated with GSKJ1 for 30 min and stimulated with IFN-γ at the indicated times. Co-IP assessment of the interaction between Stat1 and Kdm6b for the indicated times using an anti-Stat1 antibody. c, Macrophages were pretreated with GSKJ1 for 30 min and stimulated with IFN-γ at the indicated times. Left: western blot detection of Jak1, phosphorylated Jak1 (p-Jak1) and Kdm6b with Gapdh used as an endogenous control. Right: the quantified results. *P < 0.05. d, Macrophages were pretreated with GSKJ1 for 30 min and stimulated with IFN-γ at the indicated times. Left: co-IP analysis of the interaction between Jak1 and Kdm6b using an anti-Jak1 antibody. Right: the quantified results. *P < 0.05. e, Macrophages were stimulated with IFN-γ for 30 min. IF analysis of Jak1 and Kdm6b in macrophages was performed. Nuclei are visualized with DAPI. Scale bars, 50 μm (left) and 10 μm (right). f, Macrophages were stimulated with IFN-γ for 30 min with or without GSKJ1. Left: co-IP detection of the interaction between Jak1 and Kdm6b using an anti-Jak1 antibody. Right: the quantified results. *P < 0.05.

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