Fig. 5: EHMT2 targets H3K9me2 to modulate the transcription and translation of GADD45G. | Experimental & Molecular Medicine

Fig. 5: EHMT2 targets H3K9me2 to modulate the transcription and translation of GADD45G.

From: EHMT2 aggravates vascular remodeling via epigenetic inhibition of GADD45G

Fig. 5: EHMT2 targets H3K9me2 to modulate the transcription and translation of GADD45G.The alternative text for this image may have been generated using AI.

a Venn diagram showed the number of DEGs uniquely identified in each comparison. b Heatmap of the transcriptomic profiling of the DEGs. c,d KEGG (c) and GO (d) analyses of DEGs in VSMCs. e GSEA of DEGs in VSMCs treated as indicated within pathways related to cell death and growth. f Pie chart displaying the genome-wide distribution of H3K9me2 occupancy in promoter, exon, intron and intergenic regions in VSMCs derived from CUT&Tag data. g Heatmap of the target genes associated with differential H3K9me2 peak binding sites in si-EHMT2 and si-Scr VSMCs following PDGF-BB stimulation. h Venn diagram depicting the intersection of genes under EHMT2 regulation, genes bound by H3K9me2 and target genes identified via GO pathway analysis of the RNA-seq data. i Analysis of motif interactions with enriched H3K9me2 peaks at the GADD45G and Klf15 loci. j IGV analysis of H3K9me2 peaks at the GADD45G and Klf15 loci in VSMCs. k ChIP–qPCR assay was performed to detect the relative binding level of H3K9me2 at the promoter region of GADD45G in VSMCs under different treatments.

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