Fig. 6: Fatty acid trafficking visualized using a fluorescent fatty acid pulse-chase assay. | International Journal of Obesity

Fig. 6: Fatty acid trafficking visualized using a fluorescent fatty acid pulse-chase assay.

From: Interleukin-33 promotes lipolysis of adipocytes and protects male mice against obesity via activation of β-adrenergic receptor signaling

Fig. 6

Cells were pulsed with Red C12 overnight, washed, and incubated with CM for 1 h in order to allow the Red C12 to accumulate in LDs. Cells were pre-incubated with CGP 20712A for 1 h prior to IL-33 treatment. A LDs were labeled using BODIPY 493/503. (n = 3 samples/group; Scale bar: 10 μm). B Relative cellular localization of Red C12 was quantified by ImageJ. C Mitochondria were labeled using MitoTracker Green. (n = 3 samples/group; Scale bar: 10 μm). D Relative cellular localization of Red C12 was quantified by Pearson’s coefficient analysis to analyze the colocalization of mitochondria and free fatty acids. E Western blot analysis showing the expression of FABP4 (n = 3 samples/group). F FABP4 quantification was statistically analyzed using ImageJ (n = 3 samples/group). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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