Fig. 4
From: Progressive tooth pattern changes in Cilk1-deficient mice depending on Hedgehog signaling
Loss of Cilk1 in mesenchyme leads to changes in tooth pattern. a–j Sagittal sections of the maxilla (Mx) and mandible (Mn)of Krt14-Cre;Cilk1fl/fl and Wnt1-Cre;Cilk1fl/fl mice at PN 0. Krt14-Cre;Cilk1fl/fl shows a normal tooth pattern with first molar (M1) and second molar (M2). Wnt1-Cre;Cilk1fl/fl shows smaller diastemal supernumerary tooth (R2), M1 and M2. i, j R2 is clearly observed in Wnt1-Cre;Cilk1fl/fl mice by the immunofluorescent staining against Krt14. DAPI was used for counterstaining. At E16.5, X-gal–positive cells were absent in R26R control mice (k), present in the oral and dental epithelium of Krt14-Cre;R26R mice (l) and observed in the oral and dental mesenchyme of Wnt1-Cre;R26R mice (m) confirming tissue-specific Cre recombinase activity. Scale bars: a–h: 250 μm, i, j: 500 μm, k–m: 200 μm
