Fig. 7

Ptprd knockdown reverses the enhanced odontoblastic differentiation of mDPCs by March2 deficiency. a RT-qPCR shows the mRNA levels of Dmp1, Dspp and Col1a1 in mDPCs cultured in the differentiation medium for 5 d following transfection with siRNAs targeting March2 and Ptprd each or both (n = 3). b WB analysis shows the protein levels of DMP1, DSPP and COL I in mDPCs cultured for 5 d in the differentiation medium following transfection with March2 siRNA or Ptprd siRNA or both. c The increase in ALP activity in differentiation-induced cells due to March2 knockdown is partially reversed by the simultaneous knockdown of Ptprd and March2. d ARS staining indicates that March2 knockdown promotes mineralized nodule formation in differentiation-induced cells, an effect that is abolished by the simultaneous knockdown of Ptprd and March2. D, differentiation induction. The statistical difference was analyzed by one-way ANOVA with Dunnett’s post hoc test (a), where ns denotes not significant; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001