Fig. 3

Endothelial Ndst1 deficiency reduces interstitial inflammation under diabetic conditions.Kidney sections were stained for macrophages (F4/80), the chemoattractant MCP1, T-cells (CD3) and neutrophils (NimpR14). a–d Macrophage (green) influx in diabetes is increased in diabetic animals compared to health controls. However the increase is reduced in the Ndst1^f/fTie2Cre⁺ group compared to the Ndst1^f/fTie2Cre⁻ group (p < 0.05). Agrin staining (red) was used to clarify the renal histology. e–h Endothelial MCP-1 is more expressed in diabetic groups, especially in the Ndst1^f/fTie2Cre⁺ mice, despite a lower glomerular and tubulo-interstitial macrophage influx in latter group. i–j T-cells (i) and neutrophil (j) influx were unchanged between the Ndst1^f/fTie2Cre^- and Ndst1^f/fTie2Cre⁺ group. Moreover compared to healthy control animals T-cell influx was not increased in the diabetic groups. Representative photomicrographs were taken at 200× magnification