Fig. 2: Cell disruption and protein extraction efficiency using the microscale cell shredder, the Dounce Homogenizer and Qiagen Mitochondria Isolation Kit.
From: Demarcating the membrane damage for the extraction of functional mitochondria
a Cell disruption efficiency, determined by the fraction of disrupted cells against total number of intact cells, was quantified at different cell concentrations. b After the centrifugation steps, total protein yield was determined accordingly. These experiments were conducted using HEK293 cells. Results were plotted as mean ± SD (n = 3 independent experiments, **P < 0.01, ***P < 0.001)
