Fig. 6: Effect of the buffer hypertonicity.
From: Demarcating the membrane damage for the extraction of functional mitochondria
a Cell disruption efficiency, b total protein yield, and c percentage of functional mitochondria measured when the isotonic buffer and hypotonic buffer were used for mitochondrial extraction using the microscale cell shredder and the Dounce Homogenizer. Experiments were conducted by disrupting HEK293 cells of 106 cells/mL and results were plotted as mean ± SD (n = 3 independent experiments)
