Fig. 4: Cell morphology of representative neonatal human dermal fibroblasts (NHDFs) for each mesh. | Microsystems & Nanoengineering

Fig. 4: Cell morphology of representative neonatal human dermal fibroblasts (NHDFs) for each mesh.

From: Machine learning metrology of cell confinement in melt electrowritten three-dimensional biomaterial substrates

Fig. 4

NHDFs are stained for vinculin (green), actin microfilament (red), and nucleus (blue) 1 day after seeding. a(i) Concept drawing illustrating attached cells on glass coverslips used as a control culture substrate. a(ii–iii) Immunofluorescent images of representative cell morphologies obtained for the control culture substrate. b(i) Concept drawing illustrating attached cells on the SES-1 min culture substrate. b(ii–iii) Immunofluorescent images of representative cell morphologies obtained for the SES-1 min substrate. c(i) Concept drawing illustrating attached cells on the SES-3 min culture substrate. c(ii–iii) Immunofluorescent images of representative cell morphologies obtained for the SES-3 min culture substrate. 3D renderings of all depicted cell morphologies are provided in Supplemental Information

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