Fig. 3: Cell capture in microchambers. | Microsystems & Nanoengineering

Fig. 3: Cell capture in microchambers.

From: Single-cell protein profiling in microchambers with barcoded beads

Fig. 3

a Labelling of MCF-7 cells with functionalized beads (diameter: 4.5 µm). A successfully labelled cell binds to at least one magnetic bead. The labelling of >90% of beads was achieved with an incubation time of at least 15 min at a concentration of 600 µg mL−1. b The use of beads functionalized with specific antibodies allowed for the capture of mammalian (MCF-7), yeast (K. phaffii) and bacterial cells (E. coli) as well as lipid vesicles, and (c) larger target organisms yielded lower capture success

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