Fig. 2: On-chip delivery of molecular beacons (MBs) for intracellular mRNA detection.
From: On-chip multiplexed single-cell patterning and controllable intracellular delivery
a Vimentin CDS MBs were designed to probe the expression of mRNA to determine the regulation of the VIM gene in living cells. b The fluorescence observed at the single-cell level on the 3D EP chip indicates that vimentin is highly expressed in myofibroblasts. Scale bar: 5 µm. c Culturing myofibroblasts in Petri dishes verified the high expression of vimentin, as well as the presence of intact membranes after electroporation (PI dye). Scale bar: 20 µm. d The delivery uniformity of 3D EP chip and the commercial BEP, respectively. e The delivery efficiency of 3D EP chip and the commercial BEP, respectively. f The cell viability after electroporation with 3D EP chip or the commercial BEP, respectively. ***p-value < 0.005; **p-value < 0.01.
