Fig. 1: Diagram of spheroid and organoid development.

a From human cancer tissue, tumor cells can be isolated and placed in culture to yield spheroids. b Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) are two common stem cell types used as the cellular source for organoid production. Both ESCs and iPSCs can form a variety of organoid models when given the right signaling cues and ECM