Fig. 3: An example of liver organoids generated by the extracellular matrix scaffold method.

These liver organoids were achieved by seeding hepatocarcinoma, human mesenchymal, and endothelial cells in a liver-derived 3D ECM hydrogel termed LEMgel. a Phase contrast and (b) fluorescence images show live (green) and dead (red) cells within liver organoids. Reproduced with the permission of Wiley²⁸. c Schematic of the generation of kidney organoids using the spinning flask method. This particular example showed how embryoid bodies were formed from pluripotent stem cells and placed into the spinning flask to produce the kidney organoids. Reproduced with the permission of MDPI³⁴