Fig. 6: Drug treatment of cancer cells.

a Drug treatment protocol for doxorubicin assays. b Micrographs showing a single spheroid at three different drug concentrations for two PDX lines. PH039 showed sensitivity to drug treatment at 0.659 µM, while PH704 sensitivity was lower at the same concentration. Scale bar = 50 µm. c Doxorubicin IC50 curves for sensitive (PH039, IC50–0.659 µM) and resistant (PH704, IC50–4.941 µM) ovarian cancer PDX lines. Red dotted lines denote IC50 values. Black dotted lines represent the viability of the negative control at day 6 (n ≥ 5)