Fig. 4: Confirmation of 3D MGIS for long-time monitoring of 3D cell viability. | Microsystems & Nanoengineering

Fig. 4: Confirmation of 3D MGIS for long-time monitoring of 3D cell viability.

From: 3D microgroove electrical impedance sensing to examine 3D cell cultures for antineoplastic drug assessment

Fig. 4

a Bode plot of the Matrigel/cell construct with cells at different seeding densities. b A group of representative cell growth curves for 3D A549 cells with different numbers of cells ranging from 20,000 to 100,000 cells/well. c Representative cell growth curves of 3D cultured A549 cells, HepG2 cells, and MDCK cells. d Live/dead analysis on days 0 and 7 of the Matrigel/cell constructs. The top image represents the top view of the total construct; the bottom image represents a 3D reconstruction of a 100 μm Z-stack of confocal images. Green fluorescence indicates calcein-AM-stained live cells, and red fluorescence indicates PI-stained dead cells; scale bar: 200 μm. All the data are represented by the means ± SDs (standard deviations), n = 3

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