Fig. 3: Simultaneous tracking of bacterial growth kinetics in two parallel channels with two different culture conditions. | Microsystems & Nanoengineering

Fig. 3: Simultaneous tracking of bacterial growth kinetics in two parallel channels with two different culture conditions.

From: Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

Fig. 3

The growth kinetics of co-Emerald M. smegmatis cells treated with the small-molecule test drug CB7620870 compared to cells growing in normal media 7H9-ADC were compared. a M. smegmatis cells cultured in 7H9-ADC exhibited robust exponential growth with minimal cell death, as evidenced by the increase in live M. smegmatis cells expressing co-Emerald and the absence of PI-stained M. smegmatis cells during the 16 h culture period. b M. smegmatis cells treated with the small-molecule test drug CB7620870 for 29 h at 200 μM after being cultured initially in 7H9-ADC for 7 h showed significantly slower growth, with some cell death indicated by PI staining. Scale bar = 20 µm.

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