Fig. 5: Large-scale microassembly using optical tweezers. | Microsystems & Nanoengineering

Fig. 5: Large-scale microassembly using optical tweezers.

From: Assembly of multicomponent structures from hundreds of micron-scale building blocks using optical tweezers

Fig. 5

a Scanning electron microscope (SEM) image of a 3D periodic 8 × 8 × 7 simple cubic lattice consisting of alternating biotin-coated and streptavidin-coated 1 µm polystyrene spheres. Inset: 3D layout of the two components. b SEM image from (a) with overlaying spheres in red along the front, side, and top faces of the structure, which are used to estimate a mean absolute 3D positional error of 180 nm. c High-magnification SEM image of a corner of a 6 × 6 grid of alternating biotin- and streptavidin-coated 1 µm spheres. The streptavidin-coated spheres exhibit a rougher surface. d–f Optical microscope images of the full structure in (c). The biotin-coated spheres are green-fluorescent, while the streptavidin-coated spheres are red-fluorescent. The brightfield image is shown in (d), and the fluorescence image obtained using a FITC filter set is shown in (f). A mixed modality image (brightfield + fluorescence) is shown in (e).

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