Fig. 5: Identification of single and multiparticle occupancy in a GIE trap.
From: Surface-modified elastomeric nanofluidic devices for single nanoparticle trapping

Negatively charged single 80 nm diameter gold nanoparticles trapped in multiple (a) 500 nm and (b) 200 nm pockets (blue circle). Double and multioccupancy of nanoparticles was also observed (red circle) more frequently in the case of (a) 500 nm pockets than (b) 200 nm pockets. Multioccupancy was also detectable from the intensity profile of trapped nano-objects inside the nanopockets of (c) 500 nm and (d) 200 nm diameter, where the intensity was normalized with the maximum RGB value (255)