Fig. 4: Data collected and analyzed in the smartphone and the performance of this system.

a The interface of the smartphone for data collection and analysis. b Program algorithm for the statistical analysis of cell number, area, and perimeter before and after stress. c Direct comparison of RBCs concentrations calculated by the smartphone-based device and standard hemocytometer. The solid black line represents the regression line, the solid gray line represents the identity line, and two dashed red lines represent the confidence band (n = 50). d Bland–Altman analysis to compare the results obtained by the smartphone-based device and standard hemocytometer. The red dashed line is the mean difference of the methods, and the gray dashed lines represent the 95% LOAs. e ROC curves. f, g Bland–Altman analysis to compare the mean diameter, circularity obtained by the smartphone-based device and microscopic examination, and Countstar cell counter (n = 56)