Fig. 5: Effect of the experimental parameters for ELISA signals on the rotational paper-based analytical device. | Microsystems & Nanoengineering

Fig. 5: Effect of the experimental parameters for ELISA signals on the rotational paper-based analytical device.

From: A novel polymer-based nitrocellulose platform for implementing a multiplexed microfluidic paper-based enzyme-linked immunosorbent assay

Fig. 5: Effect of the experimental parameters for ELISA signals on the rotational paper-based analytical device.The alternative text for this image may have been generated using AI.

a Optimization of pipetting volume loaded into the reaction zone. b–d The effect of the incubation times (capture antibody, signal antibody and antigen) on the signal intensity. e The effect of pH on the signal intensity (n = 3)

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