Fig. 4: 2D scanned images by single-camera and dual-camera acoustofluidic scanning nanoscopes. | Microsystems & Nanoengineering

Fig. 4: 2D scanned images by single-camera and dual-camera acoustofluidic scanning nanoscopes.

From: An acoustofluidic scanning nanoscope using enhanced image stacking and processing

Fig. 4: 2D scanned images by single-camera and dual-camera acoustofluidic scanning nanoscopes.

a Image tracking result comparison between single-camera imaging and the dual-camera imaging methods. Collected information (x-, y-coordinates, radius, magnification factor) from the image of camera #1 was applied to the image of camera #2. The red missing area was detected when the single-camera imaging method was applied. b The scanning performance comparison between the single-camera and dual-camera imaging methods. Scanning performance was evaluated for the letter for each number of image frames for both imaging methods. c Scanned letter “K” images composed of different numbers of image frames from 2 to 50. The letter comprised 800 nm chrome gratings pitched at the same size. d Scanned 2D images of the letters “DUKE” by using 20 µm polystyrene microspheres with a 20× microscope objective. The red box indicates the enlarged scanning area in the letter “K”. The scale bar is 2 µm.

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