Fig. 2: Flow measurments in a circular deposition chamber.
From: Deposition chamber technology as building blocks for a standardized brain-on-chip framework
a Assessment of fluid renewal within a cylindrical deposition chamber, where the exchange of fluorescein (green) and rhodamine 6 G (red) is analyzed at different time points (0, 30 and 60 s). Scale bars indicate 250 µm. Plots indicating the measured intensities of both fluorophores as a function of time (seconds) for b 4 µL, c 40 µL, and d 80 µL of inlet infused volumes of fluorescein (green) and rhodamine 6 G (red). e Quantification of the surface coverage of a cylindrical deposition chamber by neurons as a function of deposition time (seconds) for various inlet infused volumes. Solid lines indicate the model prediction for each condition, and discontinuous lines represent the surface coverage at (e.i) 35 s, (e.ii) 100 s, and (e.iii) 185 s after volume infusion. Transmission light microscopy images, and scale bars indicate 200 µm. f Quantification of the surface coverage of a cylindrical deposition chamber by neurons as a function of deposition time (seconds) based on the division of the chamber in four quarters, which are illustrated in panel g. Scale bar indicates 200 µm
