Fig. 5: Modeling of vascularized tumor spheroid.
From: U-IMPACT: a universal 3D microfluidic cell culture platform
a Timeline of the vasculogenesis model. The mixture of LFs, ECs, and hydrogel was injected into the MC. After 4 days, the EC suspension was added to both side channels. b Confocal imaging of the perfusable vascular network (green) on Day 6. Scale bar, 50 μm. c Timeline of the vascularized tumor model. The mixture of LFs, ECs, a tumor spheroid, and hydrogel was injected into the MC. After 4 days, the EC suspension was added to both side channels. d Simulation of growth factor concentration in the U-IMPACT. The tumor spheroid was the main source of growth factors. e Distribution of growth factor concentration in the middle of the U-IMPACT (A-A′). f Confocal imaging of vascularized tumor spheroid (green, U87MG; red, EC). Scale bar, 250 μm. g Imaging of sectioned tumor spheroid (left, immunofluorescence; right, bright field; blue, DAPI; green, U87MG; red, EC). Scale bar, 100 μm.
