Fig. 6: 3D tumor and neural cell culture model in the U-IMPACT.

a Confocal imaging of angiogenesis toward UC in which tumor cells and LF were seeded. We used three types of tumor spheroids composed of U87MG, HCT116, and SW 480 cells. Scale bar, 250 μm. b Confocal imaging of vascularized tumor spheroid. Scale bar, 400 μm. c Timeline (left) and confocal imaging (right) of tumor cell migration. We injected collagen type I gel into the MC and seeded the tumor cell suspension in the side channels. We then injected 2% FBS in the medium through the first inlet on Day 2. Tumor cells (green) migrated toward the first inlet. Scale bar, 500 μm. d Timeline (left) and confocal imaging (right) of neurosphere with iNSCs. We injected the neurosphere and hydrogel (Matrigel) into the MC. The iNSCs differentiated into neurons and oligodendrocytes (Tuj1, orange; Oligo2, green; DAPI, blue). Scale bar, 250 μm.