Fig. 3: SEM images and accompanying schematic demonstrating the facile surface modification for antibody-mediated analyte detection.

a SEM image of the Ti/Au IDE on the PC surface, along with a 3D microelectrode structure. b SEM image of the IDE with a higher magnification, showing the conjugated antibodies on the surface. c AFM imaging and schematic illustration of the image shown in b. (i) The functional Au IDE surface before antibodies are added. (ii) The surface of the IDE after antibody conjugation. (iii) A schematic illustration showing the overall fabrication of the IDE on PC as the base substrate, followed by a mixed-air plasma treatment, Ti seed layer, Au functional layer, and then antibody conjugation facilitated by a supplemental mixed-air plasma treatment