Fig. 5: Electrochemical impedimetric characterization of the base IDE fabrication at differential temperatures, utilizing the attached microheater to vary the DPBS temperatures.
a Compilation of all impedimetric temperature recordings across the (22–42 °C) temperature range. b Selected temperatures (every 5 °C) from a, including fitted models for each, derived from the equivalent circuit shown in the inset. c Enhanced inset data from b, showing the 1–4 MHz region of interest, where changes in the faradaic reactions at the electrode surface are apparent. Here, a clear decreasing impedance trend is observed, consistent with a hypothesis of increased kinetic activity within the electrolyte solution. d Nyquist plot variation of the data from b and c which plots imaginary impedance against real impedance. The curve shown corresponds to the first faradaic reactions governed by the start of the equivalent circuit, present at the higher frequencies indicated. The highlighted region of the curve is governed primarily here by R1* and R2* and confirms the same trend of decreasing real impedance observed in c
