Fig. 7: COMSOL microfluidic modeling of the device with a constant 8 Pa applied pressure.

The timepoints are 0 ms, 200 ms, 500 ms, and 1 s post application. These time points are reflective of the localized application of the simulated l-glutamine. The inset images are enlargements of a port at each timepoint to better visualize the concentration changes (scale bars are for the inset images only). At 0 ms, there is no substantial ingress of the simulated 0.2 mM l-glutamine solution. At 200 ms and at 500 ms, the solution is still highly localized near the inlet. At 1 s, the solution is still localized, however this timepoint is the maximal time at which pressure should be applied to retain local chemical stimulation. Images of timepoints beyond this 1 s time are shown in Supplementary Fig. S4