Fig. 5: Performance results of the leukocyte preparation methods.

a–c Microphotographs of cell samples before treatment (i) and recovered after RBC lysis, 3 and 5 μm micropore membrane-based filtration operations (ii, iii). d The proportion of surviving cells in leukocytes after cell sample preparation. e RBC residual rate and f PLT residual rate in cell samples after preparation. g The proportion of leukocytes after cell sample preparation. The datas are shown as the means ± SEMs and compared by unpaired Student’s t test or one-way analysis of variance (ANOVA). *P < 0.05, **P < 0.01; NS: no significance