Fig. 3: The valve diffusion analysis using the colour intensity evolution.
From: Diffusion-free valve for preprogrammed immunoassay with capillary microfluidics
a Color intensity during π-valve loading and activation. Solid red and blue lines are the color intensities of the red and blue liquids at position \({P}_{2}\), respectively. The dotted blue line is the color intensity of the blue liquid at position \({P}_{1}\). b Picture of the valve during activation. The scale bar is 1 mm. c Color intensity during activation at position 2 (\({P}_{2}\)). The solid lines are the average intensity at 20 Hz, and the dashed lines are their respective standard deviations. Background noise is subtracted by selecting a second ROI close to the detection site. Intensities are taken from Video 3 (SI), which shows the activation and release of the 3D printed \(\pi\)-valve
