Fig. 6: Double-stranded ctDNA capture strategy in biological samples. | Microsystems & Nanoengineering

Fig. 6: Double-stranded ctDNA capture strategy in biological samples.

From: High-throughput extraction on a dynamic solid phase for low-abundance biomarker isolation from biological samples

Fig. 6

Schematics of the double-stranded ctDNA capture strategy in biological samples. Step 1: The sample containing the target DNA and biotinylated capture probes are mixed. Step 2: Heating at 95 °C denatures the double-stranded DNA. Step 3: Decrease the temperature until the capture probe annealing temperature to specifically hybridize each target ssDNA to the biotinylated capture probes. Step 4: After capture probes are annealed to the target DNA, the sample is injected into the device, which is filled with streptavidin-coated magnetic beads, at room temperature. High streptavidin–biotin affinity will result in target DNA capture onto the beads. Step 5: DNA detection and quantification is done either by fluorescence measurements, qPCR, or LCR assays (pictures created using BioRender application)

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