Fig. 7: Influence of the cooling rate on the capture efficiency of a double-stranded ctDNA sequence in the fluidized bed. | Microsystems & Nanoengineering

Fig. 7: Influence of the cooling rate on the capture efficiency of a double-stranded ctDNA sequence in the fluidized bed.

From: High-throughput extraction on a dynamic solid phase for low-abundance biomarker isolation from biological samples

Fig. 7

Capture efficiencies of the fluorescently mutant dsDNA sequence of 80 bp of the BRAF gene after hybridization at different cooling rates: −0.1, −0.05, and −0,08 °C/s up RT. ctDNA was prepared at 10 nM in 10 mM Tris-HCL buffer. Capture was performed in an FB chip at 15 µL/min with vibration and bimodal support (p > 0.05)

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