Fig. 8: Detection of LCR products by gel electrophoresis after capture of dsDNA BRAF target in serum samples in the fluidized bed. | Microsystems & Nanoengineering

Fig. 8: Detection of LCR products by gel electrophoresis after capture of dsDNA BRAF target in serum samples in the fluidized bed.

From: High-throughput extraction on a dynamic solid phase for low-abundance biomarker isolation from biological samples

Fig. 8

Detection of 1 pM (6 × 105 copies/µL) (left) and 100 fM (6 × 104 copies/µL) (right) dsDNA 277 bp BRAF V600E target by gel electrophoresis (2% agarose gel) of LCR products (white arrows) after capture in serum samples by fluidized bed. LCR probes are represented by white dotted arrows. Left -> Right: Ladder, LCR on-beads a LCR of heat-released target b. The expected product size is 100 bp

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