Fig. 2: Workflow of the modular microfluidic device and experimental setup for on-chip and off-chip analysis of ER+ breast cancer cells exposed to FSS. | Microsystems & Nanoengineering

Fig. 2: Workflow of the modular microfluidic device and experimental setup for on-chip and off-chip analysis of ER+ breast cancer cells exposed to FSS.

From: A modular microfluidic platform to study how fluid shear stress alters estrogen receptor phenotype in ER+ breast cancer cells

Fig. 2

a Top view of the shearing device consisting of a 1 m long channel that is 70 μm wide and 100 μm tall. b Top and side view of the microwell trapping array consisting of a 3000-member array of 40 ×40 μm wide and 40 μm deep traps used for single cell analysis as well as a representative brightfield image of MCF-7 cells after being sheared and trapped. c Depiction of a microtube used to collect cells for off-chip analysis which are then pelleted and analyzed by western blot. d Picture of the shearing module with red dye in the fluidic channel. e Picture of the microwell trapping array with red dye in the channel. f Real setup of an on-chip analysis experiment mounted on the microscope. g Real setup of an off-chip analysis experiment showing multiple shearing modules depositing cells into microtubes

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